POLB antibody (acLys72)

Catalog No. ABIN8123855

This Rabbit Polyclonal antibody specifically detects POLB in WB and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for POLB antibody (acLys72) (ABIN8123855)

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLB Antibody

Binding Specificity: acLys72

Purpose: DNA pol β (Acetyl Lys72) Antibody

Specificity: This antibody detects endogenous levels of Human,Mouse,Rat DNA pol β (Acetyl Lys72)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human DNA pol beta (Acetyl Lys72)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: DNA pol beta

Background:

Synonyms: DNA polymerase beta (EC 2.7.7.7, EC 4.2.99.-)

Background: catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Binds 2 magnesium ions per subunit.,domain:Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.,function:Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.,PTM:Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.,similarity:Belongs to the DNA polymerase type-X family.,subunit:Monomer.,

Gene Name: POLB

Protein Name: DNA pol β (Acetyl Lys72)

Gene ID: 5423

UniProt: P06746

Pathways: DNA Damage Repair