ALIX antibody

Catalog No. ABIN8123960

This Rabbit Monoclonal antibody specifically detects ALIX in WB, IHC, IF, ELISA and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ALIX antibody (ABIN8123960)

Target: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ALIX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP)

Clone: AB1695

Product Details anti-ALIX Antibody

Purpose: ALIX Rabbit mAb

Specificity: This antibody detects endogenous levels of ALIX protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for ALIX

Target: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Alternative Name: ALIX

Background:

Synonyms: Programmed cell death 6-interacting protein (PDCD6-interacting protein, ALG-2-interacting protein 1, Hp95)

Background: This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012],

Gene Name: PDCD6IP AIP1 ALIX KIAA1375

Protein Name: ALIX

Gene ID: 10015

UniProt: Q8WUM4

Pathways: p53 Signaling, EGFR Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Tube Formation