HSD11B1 antibody (Internal Region)

Catalog No. ABIN768641

The Goat Polyclonal anti-HSD11B1 antibody is suitable to detect HSD11B1 in samples from Mouse. It has been validated for WB and ELISA.

Quick Overview for HSD11B1 antibody (Internal Region) (ABIN768641)

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This HSD11B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-HSD11B1 Antibody

Binding Specificity: Internal Region

Purpose: Hsd11b1 (mouse)

Sequence: TARSEEGLQK VVSR

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TARSEEGLQKVVSR, from the internal region of the protein sequence according to NP_032314.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 32 kDa band observed in Mouse Lung lysates (calculated MW of 32.4 kDa according to NP_032314.2). Recommended concentration: 0.3-1 μg/mL. An additional band of unknown identity was also consistently observed at 45 kDa. This band was succe

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HSD11B1

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Alternative Name: Hsd11b1

Background: Hsd11b1, hydroxysteroid (11-beta) dehydrogenase 1, 11-DH, 11-beta-HSD1, HDL, HSD11, HSD11B, HSD11L, 11-beta-hydroxysteroid dehydrogenase 1, corticosteroid 11-beta-dehydrogenase isozyme 1, short chain dehydrogenase/reductase family 26C, member 1

Molecular Weight: 32.4kDa according to NP_032314.2

Gene ID: 15483, 25116

NCBI Accession: NP_032314

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Carbohydrate Metabolic Process