MYO5A antibody (AA 1063-1072)

Catalog No. ABIN793167

Product Details anti-MYO5A Antibody

Target: MYO5A (Myosin VA (MYO5A))

Binding Specificity: AA 1063-1072

Reactivity: Human, Mouse, Rat, Cow, Bat, Chicken, Dog, Hamster, Horse, Monkey, Pig, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This MYO5A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Human MYO5A. This antibody is expected to recognize both reported isoforms (NP_000250.3, NP_001135967.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%) Xenopus (90%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-ETKQLELDLN from an internal region of human MYO5A (NP_000250.3, NP_001135967.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%), Xenopus (90%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IF, Peptide-ELISA (1:8000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 200kD band observed in lysates of cell line Jurkat (calculated MW of 215kD according to NP_000250.3). Recommended concentration: 1-3 μg/mL. Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fiber membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Rder et al, Proc Natl Acad Sci U S A. 2010 Feb 2,107(5):2031-6, PMID: 20133847). Recommended concentration: 1-3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MYO5A

Target: MYO5A (Myosin VA (MYO5A))

Alternative Name: MYO5A / Myosin V (MYO5A Products)

Synonyms: myo5a antibody, MGC53270 antibody, si:dkey-266j9.1 antibody, MYOA antibody, MYO5A antibody, D antibody, Dop antibody, Myh12 antibody, 9630007J19Rik antibody, AI413174 antibody, AI661011 antibody, Dbv antibody, MVa antibody, Myo5 antibody, MyoVA antibody, Sev-1 antibody, d antibody, d-120J antibody, flail antibody, flr antibody, DILUTE antibody, myoxin) antibody, non-muscle antibody, GS1 antibody, MYH12 antibody, MYO5 antibody, MYR12 antibody, myosin VA L homeolog antibody, myosin VAa antibody, myosin VA antibody, myosin va antibody, myo5a.L antibody, myo5aa antibody, MYO5A antibody, PY04789 antibody, Myo5a antibody

Background: Name/Gene ID: MYO5A

Synonyms: MYO5A, GS1, Myosin heavy chain 12, Myosin V, Myosin-12, MYO5, Myoxin, MYR12, MYH12, Myosin-Va, Unconventional myosin-Va

Gene ID: 4644

NCBI Accession: NP_000250, NP_001135967

UniProt: Q9Y4I1

Pathways: Hormone Transport, Peptide Hormone Metabolism