IFNAR2 antibody (Carboxyfluorescein (CFS))

Catalog No. ABIN796985

The Goat Polyclonal anti-IFNAR2 antibody has been validated for FACS. It is suitable to detect IFNAR2 in samples from Mouse.

Quick Overview for IFNAR2 antibody (Carboxyfluorescein (CFS)) (ABIN796985)

Target: IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IFNAR2 antibody is conjugated to Carboxyfluorescein (CFS)

Application: Flow Cytometry (FACS)

Product Details anti-IFNAR2 Antibody (Carboxyfluorescein (CFS))

Specificity: Mouse IFNA/B R2.

Purification: Immunoaffinity purified

Immunogen: Mouse myeloma cell line NS0- derived recombinant human IFN-a/b R2 extracellular domain

Isotype: IgG

Application Details

Application Notes: Approved: Flo

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Assay Procedure:

Flow Cytometry Validation
This antibody has been tested for flow cytometry using A20 cells.

1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at room temperature. Do not wash excess blocking IgG from this reaction.
   
2. After blocking, 10ul of conjugated antibody was added to 1 - 2.5 x 10e5 cells and incubated for 30 minutes at room temperature.
   
3. Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer.
   
4. The cells were resuspended in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with CFS-labeled goat IgG antibody. This procedure may need to be modified, depending upon the cell type and final utilization. Individual users may need to titrate to determine the optimal reagent amount for their specific use.
   
5. Cell surface expression of IFN-a/b R2 was determined by flow cytometry using 488 nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 515 - 545 nm.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, up to 0.5 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles and prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 4°C for up to 1 year.

Expiry Date: 12 months

Target Details for IFNAR2

Target: IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))

Alternative Name: IFNAR2

Background: Name/Gene ID: IFNAR2
Family: Interferon Receptor

Synonyms: IFNAR2, IFN-alpha/beta receptor 2, IFN-alpha-REC, IFN-alpha binding protein, IFN-R, IFN-R-2, IFNARB, Type I interferon receptor 2, IFNABR

Gene ID: 3455

UniProt: P48551

Pathways: JAK-STAT Signaling, Hepatitis C