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HDEL antibody (C-Term)

HDEL Reactivity: Saccharomyces cerevisiae WB, IF, ICC Host: Mouse Monoclonal 2E7 unconjugated
Catalog No. ABIN863081
  • Target See all HDEL products
    HDEL
    Binding Specificity
    C-Term
    Reactivity
    • 22
    • 12
    • 11
    • 10
    • 10
    • 10
    • 10
    • 10
    • 1
    Saccharomyces cerevisiae
    Host
    • 22
    Mouse
    Clonality
    • 22
    Monoclonal
    Conjugate
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This HDEL antibody is un-conjugated
    Application
    • 22
    • 12
    • 10
    • 10
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Detects ~78 kDa.
    Cross-Reactivity
    Drosophila melanogaster, Plant, Saccharomyces cerevisiae
    Purification
    Protein G Purified
    Immunogen
    Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
    Clone
    2E7
    Isotype
    IgG2b
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN863080 was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Target
    HDEL
    Alternative Name
    HDEL (HDEL Products)
    Background
    HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
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