E-cadherin antibody (AA 662-675)

Catalog No. ABIN870690

The Goat Polyclonal anti-E-cadherin antibody (ABIN870690) specifically detects E-cadherin in WB, IHC and ELISA. The antibody is reactive with Human, Mouse, Rat and Pig samples.

Quick Overview for E-cadherin antibody (AA 662-675) (ABIN870690)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-E-cadherin Antibody

Binding Specificity: AA 662-675

Purpose: CDH1 (aa662-675)

Sequence: DYKINLKLMD NQNK

Specificity: The immunizing peptide represents part of the extracellular domain.

Cross-Reactivity: Dog, Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DYKINLKLMDNQNK, from the internal region of the protein sequence according to NP_004351.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thyroid. Recommended concentration: 5 μg/mL.

Western Blot: Approx 85 kDa band observed in Human Kidney lysates and in Pig Colon, Kidney and Lung lysates and ~90 kDa in Mouse and Rat Lung lysates (calculated MW of 97.5 kDa according to NP_004351.1). Recommended concentration: 0.3-1 μg/mL. Primary incub

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CDH1

Background: CDH1, cadherin 1, type 1, E-cadherin (epithelial), Arc-1, CD324, CDHE, ECAD, LCAM, UVO, CAM 120/80, E-Cadherin, cadherin 1, E-cadherin (epithelial), cadherin-1, calcium-dependent adhesion protein, epithelial, cell-CAM 120/80, epithelial cadherin, uvomorul

Molecular Weight: 97.5kDa

Gene ID: 999, 83502

NCBI Accession: NP_004351

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation