PPM1F antibody (AA 325-425)

Catalog No. ABIN872949

Product Details anti-PPM1F Antibody

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Binding Specificity: AA 325-425

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPM1F antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PPM1F

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PPM1F

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Alternative Name: PPM1F (PPM1F Products)

Synonyms: 1110021B16Rik antibody, 4933427B07Rik antibody, CaMKPase antibody, Popx2 antibody, mKIAA0015 antibody, CAMKP antibody, FEM-2 antibody, POPX2 antibody, hFEM-2 antibody, protein phosphatase, Mg2+/Mn2+ dependent 1F antibody, protein phosphatase 1F (PP2C domain containing) antibody, protein phosphatase, Mg2+/Mn2+ dependent, 1F antibody, PPM1F antibody, Ppm1f antibody

Background:

Synonyms: Ca2+/calmodulin-dependent protein kinase phosphatase, CaM-kinase phosphatase,CaMKPase, hFEM-2, KIAA0015, Partner of PIX 2, POPX2, Protein phosphatase 1F, PPM1F_HUMAN.

Background: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008].

Gene ID: 9647

Pathways: Positive Regulation of Endopeptidase Activity