SHP1 antibody (pTyr536) (AbBy Fluor® 647)

Catalog No. ABIN879862

This Rabbit Polyclonal antibody specifically detects SHP1 in WB, FACS, IF (cc) and IF (p). It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Quick Overview for SHP1 antibody (pTyr536) (AbBy Fluor® 647) (ABIN879862)

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHP1 antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-SHP1 Antibody (AbBy Fluor® 647)

Binding Specificity: pTyr536

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Dog,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PTPN6 around the phosphorylation site of Tyr536 [SE(p-Y)GN]

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-SHP1 antibody (AbBy Fluor® 647) (ABIN879862)

Lopes, Bálint, Valvo, Felce, Hessel, Dustin, Davis: "Membrane nanoclusters of FcγRI segregate from inhibitory SIRPα upon activation of human macrophages." in: The Journal of cell biology, Vol. 216, Issue 4, pp. 1123-1141, (2017) (PubMed).

Target Details for SHP1

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Alternative Name: PTPN6

Background:

Synonyms: SHP-1Tyr536, PTPN6phospho Y536, SHP1phospho Y536, 70 kda SHP1L protein, HCP, HCPH, Hematopoietic cell phosphatase, Hematopoietic cell protein tyrosine phosphatase, Hematopoietic cell protein-tyrosine phosphatase, HPTP1C, Protein tyrosine phosphatase 1C, Protein tyrosine phosphatase non receptor type 6, Protein tyrosine phosphatase SHP1, PTP1C, PTPN6, SH PTP 1, SHP1, SHP1L, PTN6_HUMAN.

Background: SHP1 is a member of the non receptor protein tyrosine phosphatase subfamily. The PTP family comprises of at least 37 proteins, characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. PTP1B is cytosolic. The PTPs have high substrate specificity for phosphotyrosyl proteins, at the primary sequence level sharing little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. SHP1 is implicated in the control of tyrosine kinase signalling pathways in cellular proliferation, with a potential role in cancer.

Gene ID: 5777

UniProt: P29350

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling