Caspase 8 antibody (AA 411-482) (AbBy Fluor® 647)

Catalog No. ABIN886653

This anti-Caspase 8 antibody is a Rabbit Polyclonal antibody detecting Caspase 8 in WB, FACS, IF (cc) and IF (p). Suitable for Human, Mouse, Rat, Monkey, Pig and Sheep.

Quick Overview for Caspase 8 antibody (AA 411-482) (AbBy Fluor® 647) (ABIN886653)

Target: Caspase 8 (CASP8)

Reactivity: Human, Mouse, Rat, Monkey, Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Caspase 8 Antibody (AbBy Fluor® 647)

Binding Specificity: AA 411-482

Cross-Reactivity: Human, Monkey, Mouse, Pig, Rat, Sheep

Predicted Reactivity: Dog,Cow,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat CASP8 subunit p10

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase 8

Background:

Synonyms: MACH, Mch5, FLICE, CASP-8, Caspase-8, Casp8

Background: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP1. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.

Gene ID: 12370

UniProt: Q9JHX4

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades