KDEL antibody (Cy5)
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- Target See all KDEL (Lys Asp Glu Leu) products
- KDEL (Lys Asp Glu Leu)
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Reactivity
- Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This KDEL antibody is conjugated to Cy5
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Application
- Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Cross-Reactivity
- Mouse, Rat
- Predicted Reactivity
- Human,Dog,Cow,Pig,Rabbit,Guinea Pig
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide KDEL
- Isotype
- IgG
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- Application Notes
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FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- KDEL (Lys Asp Glu Leu)
- Abstract
- Lys Asp Glu Leu Products
- Background
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Synonyms: KDEL, Lys Asp Glu Leu antibody, GRP 78, GRP 94, KDEL1_HUMAN.
Background: Endoplasmic reticulum Marker. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
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