MBD1 antibody (AA 101-200) (Alexa Fluor 350)

Catalog No. ABIN905807

Product Details anti-MBD1 Antibody (Alexa Fluor 350)

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Binding Specificity: AA 101-200

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD1 antibody is conjugated to Alexa Fluor 350

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MBD1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MBD1

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Alternative Name: MBD1 (MBD1 Products)

Synonyms: CXXC3 antibody, PCM1 antibody, RFT antibody, Cxxc3 antibody, CCDC11 antibody, methyl-CpG binding domain protein 1 antibody, MBD1 antibody, Mbd1 antibody

Background:

Synonyms: CXXC 3, CXXC3, MBD 1, MBD1, MECP1 COMPLEX, Methyl CpG binding domain protein 1, Methyl CpG binding domain protein 1 isoform PCM1, Methyl CpG binding protein MBD1, Methyl CpG binding protein splice variant 1, Methyl CpG binding protein splice variant 2, Methyl CpG binding protein splice variant 3, Methyl CpG binding protein splice variant 4, PCM 1, PCM1, Protein containing methyl CpG binding domain 1, Regulator of fibroblast growth factor 2 FGF 2 transcription, RFT, The regulator of fibroblast growth factor 2FGF 2 transcription.

Background: DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Gene ID: 4152