DNAJA3 antibody (AA 101-200) (Biotin)

Catalog No. ABIN917702

Product Details anti-DNAJA3 Antibody (Biotin)

Target: DNAJA3 (DnaJ (Hsp40) Homolog, Subfamily A, Member 3 (DNAJA3))

Binding Specificity: AA 101-200

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJA3 antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TID1/HCA57

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for DNAJA3

Target: DNAJA3 (DnaJ (Hsp40) Homolog, Subfamily A, Member 3 (DNAJA3))

Alternative Name: HCA57 (DNAJA3 Products)

Synonyms: Tid-1 antibody, 1200003J13Rik antibody, 1810053A11Rik antibody, C81173 antibody, Tid1l antibody, HCA57 antibody, TID1 antibody, hTID-1 antibody, DnaJ heat shock protein family (Hsp40) member A3 antibody, Dnaja3 antibody, DNAJA3 antibody

Background:

Synonyms: DnaJ Hsp40 homolog subfamily A member 3, DnaJ homolog subfamily A member 3 mitochondrial precursor, DnaJ protein Tid 1, DnaJ protein Tid1, HCA57, Hepatocellular carcinoma associated antigen 57, Highly similar to HYPOTHETICAL 105.9 KD PROTEIN F22B7.5 IN CHROMOSOME, hTid 1, hTid1, III [Caenorhabditis elegans], TID1, Tumorous imaginal discs Drosophila homolog, Tumorous imaginal discs protein Tid56 homolog, DNJA3_HUMAN.

Background: TID1 is a human homolog of the Drosophila tumor suppressor lethal tumerous imaginal discs and encodes two mitochondrial matrix localized splice variants of human Tid1 designated hTid1S and hTid1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperons for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid1L increases apoptosis induced by DNA damaging agents as mitomycin C and TNF alpha. A J domain mutant of hTid1L can dominantly suppress apoptosis and in sharp contrast the J domain mutant of hTid1S increases apoptosis. Expression of hTid1S and hTid1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti and pro apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.

Gene ID: 9093

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity