PCNA antibody (C-Term)

Catalog No. ABIN925999

This Rabbit Polyclonal antibody specifically detects PCNA in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow and Xenopus laevis.

Quick Overview for PCNA antibody (C-Term) (ABIN925999)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PCNA Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Frog, Zebrafish (Brachydanio rerio)

Immunogen: PCNA antibody was raised in rabbit using the C terminal of PCNA as the immunogen

Application Details

Application Notes: WB: 1.25 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

PCNA Blocking Peptide, , is also available for use as a blocking control in assays to test for specificity of this PCNA antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 100 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: PCNA is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. Synonyms: Polyclonal PCNA antibody, Anti-PCNA antibody, proliferating cell nuclear antigen antibody, MGC8367 antibody.

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy