CDK8 antibody (C-Term)

Catalog No. ABIN926004

This anti-CDK8 antibody is a Rabbit Polyclonal antibody detecting CDK8 in WB. Suitable for Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Chicken and Xenopus laevis.

Quick Overview for CDK8 antibody (C-Term) (ABIN926004)

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDK8 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Zebrafish (Brachydanio rerio), Frog, Chicken

Immunogen: CDK8 antibody was raised in rabbit using the C terminal of CDK8 as the immunogen

Application Details

Application Notes: WB: 1.25 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

CDK8 Blocking Peptide, , is also available for use as a blocking control in assays to test for specificity of this CDK8 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 100 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for CDK8

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Alternative Name: CDK8

Background: The protein encoded by CDK8 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. Synonyms: Polyclonal CDK8 antibody, Anti-CDK8 antibody, cyclin-dependent kinase 8 antibody.

Pathways: Cell Division Cycle, Regulation of Lipid Metabolism by PPARalpha