CDK8 antibody (AA 10-90)

Catalog No. ABIN7214134

The Rabbit Polyclonal anti-CDK8 antibody is suitable to detect CDK8 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for CDK8 antibody (AA 10-90) (ABIN7214134)

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CDK8 Antibody

Binding Specificity: AA 10-90

Purpose: Rabbit Anti-Cdk8 Polyclonal Antibody

Specificity: Cdk8 Polyclonal Antibody detects endogenous levels of Cdk8 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Cdk8 at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CDK8

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Alternative Name: Cdk8

Background: CDK8, Cyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein kinase K35Cyclin dependent kinase 8 encoded by CDK8 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.

Molecular Weight: 53kD

Gene ID: 1024

UniProt: P49336

Pathways: Cell Division Cycle, Regulation of Lipid Metabolism by PPARalpha