AIF antibody (Middle Region)

Catalog No. ABIN926871

The Rabbit Polyclonal anti-AIF antibody (ABIN926871) specifically detects AIF in WB. The antibody is reactive with Human, Rat, Mouse, Cow, Dog, Zebrafish (Danio rerio) and Chicken samples.

Quick Overview for AIF antibody (Middle Region) (ABIN926871)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivity: Human, Rat, Mouse, Cow, Dog, Zebrafish (Danio rerio), Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AIF Antibody

Binding Specificity: Middle Region

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Zebrafish (Brachydanio rerio), Chicken

Purification: Purified

Immunogen: AIFM1 antibody was raised in rabbit using the middle region of AIFM1 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

AIFM1 Blocking Peptide, , is also available for use as a blocking control in assays to test for specificity of this AIFM1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIFM1

Background: AIFM1 is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this protein induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene. Synonyms: Polyclonal AIFM1 antibody, Anti-AIFM1 antibody, apoptosis-inducing factor, mitochondrion-associated, 1 antibody, AIF antibody, MGC111425 antibody, PDCD8 antibody.

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect