Insulin Receptor antibody (Middle Region)

Catalog No. ABIN926933

The Rabbit Polyclonal anti-Insulin Receptor antibody has been validated for WB and IHC. It is suitable to detect Insulin Receptor in samples from Human, Mouse, Rat, Dog, Rabbit, Zebrafish (Danio rerio) and Xenopus laevis.

Quick Overview for Insulin Receptor antibody (Middle Region) (ABIN926933)

Target: Insulin Receptor (INSR)

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Zebrafish (Danio rerio), Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Insulin Receptor Antibody

Binding Specificity: Middle Region

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Rabbit, Zebrafish (Brachydanio rerio), Frog

Purification: Purified

Immunogen: INSR antibody was raised in rabbit using the middle region of INSR as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

INSR Blocking Peptide, (ABIN5614204), is also available for use as a blocking control in assays to test for specificity of this INSR antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: INSR

Background: This receptor binds insulin and has a tyrosine-protein kinase activity. Isoform Short has a higher affinity for insulin. INSR mediates the metabolic functions of insulin. INSR binding to insulin stimulates association of the receptor with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K). INSR can activate PI3K either directly by binding to the p85 regulatory subunit, or indirectly via IRS1. When present in a hybrid receptor with IGF1R, it binds IGF1. Synonyms: Polyclonal INSR antibody, Anti-INSR antibody, insulin receptor antibody, CD220 antibody, HHF5 antibody.

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity