GFP antibody (N-Term)

Catalog No. ABIN933569

The Mouse Monoclonal anti-GFP antibody has been validated for WB, ELISA, IP and DB. It is suitable to detect GFP in samples from Aequorea victoria.

Quick Overview for GFP antibody (N-Term) (ABIN933569)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Clone: GF28R

Product Details anti-GFP Antibody

Binding Specificity: N-Term

Purification: Protein A affinity chromatography

Immunogen: GFP antibody was raised in mouse using GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates as the immunogen.

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000-3000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Green Fluorescent Protein (GFP) tagging provide an excellent means for monitoring gene expression and protein localization in living cells, so that it is widely accepted by molecular and cell biological research. Monoclonal anti-GFP antibody can detect GFP and its variants on Western blotting, Immunoprecipitation and Immunocytochemistry. Synonyms: Monoclonal GFP antibody, Anti-GFP antibody, Green Fluorescent Protein antibody, GFP tag antibody.