Eli Nef antibody (FITC)
Reactivity: Human Immunodeficiency Virus (HIV)
ELISA, WB
Host: Mouse
Monoclonal
3F2
FITC
-
- Target
- Eli Nef
- Reactivity
- Human Immunodeficiency Virus (HIV)
- Host
- Mouse
- Clonality
- Monoclonal
- Conjugate
- FITC
- Application
- ELISA, Western Blotting (WB)
- Immunogen
- HIV1 Eli Nef antibody was raised in mouse using HIV1 Eli Nef as the immunogen.
- Clone
- 3F2
- Isotype
- IgG1
-
- Application Notes
-
ELISA: 1:500-1:1000, WB: 1:500-1:1000
Optimal conditions should be determined by the investigator. - Comment
-
Reacts with purified, whole virus nef (HIV-1 ELI) in ELISA and Western Blot. The antibody is not epitope mapped.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied liquid in PBS buffer. The FITC is attached to the free amine group.
- Precaution of Use
- Not tested for sterility or endotoxin content.
- Handling Advice
-
Avoid repeated freeze/thaw cycles.
Product is photosensitive and should be protected from light.
Dilute only prior to immediate use. - Storage
- -80 °C
- Storage Comment
- Store frozen product at -70 °C or lower.
-
- Target
- Eli Nef
- Target Type
- Viral Protein
- Background
- Nef (Negative Regulatory Factor) is a protein expressed by primate lentiviruses. These include human immunodeficiency viruses (HIV-1 and HIV-2) and simian immunodeficiency virus (SIV). Nef is one of many pathogen-expressed proteins, known as virulence factors, which function to manipulate the host's cellular machinery and thus allow infection, survival or replication of the pathogen. Nef stands for Negative Factor. Synonyms: Monoclonal HIV1 Nef antibody, Anti-HIV1 Nef antibody, HIV-1 Nef antibody, 3'ORF antibody, ABIN antibody, ABIN1 antibody, C terminal core protein antibody, F protein antibody, Nef antibody, Negative factor antibody, p27 antibody, VAN antibody.
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