His Tag antibody

Catalog No. ABIN933979

This Mouse Monoclonal antibody specifically detects His Tag in WB, ELISA, IP and DB.

Quick Overview for His Tag antibody (ABIN933979)

Target: His Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Clone: HIS-H8

Product Details anti-His Tag Antibody

Sequence: HHHHHH

Purification: Protein A affinity chromatography

Immunogen: His Tag antibody was raised in mouse using HHHHHH (6 x His) synthetic peptide conjugated to KLH as the immunogen.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:1000-3000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.

Target Details for His Tag

Target: His Tag

Target Type: Tag

Background: A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. The tag was invented by Roche. His-tags are useful in a wide variety of processes including protein purification and binding assays. Synonyms: Monoclonal His Tag antibody, Anti-His Tag antibody, 6XHis antibody, Polyhistidine Tag antibody, HIS6 Tag antibody, 6-His antibody, HHHHHH antibody.