His Tag antibody
-
- Target See all His Tag Antibodies
- His Tag
- Reactivity
- Please inquire
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This His Tag antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)
- Sequence
- HHHHHH
- Purification
- Protein A affinity chromatography
- Immunogen
- His Tag antibody was raised in mouse using HHHHHH (6 x His) synthetic peptide conjugated to KLH as the immunogen.
- Clone
- HIS-H8
- Isotype
- IgG2b
- Top Product
- Discover our top product His Tag Primary Antibody
-
-
- Application Notes
-
WB: 1:1000-3000
Optimal conditions should be determined by the investigator. - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
-
Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.
-
- Target
- His Tag
- Abstract
- His Tag Products
- Target Type
- Tag
- Background
- A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. The tag was invented by Roche. His-tags are useful in a wide variety of processes including protein purification and binding assays. Synonyms: Monoclonal His Tag antibody, Anti-His Tag antibody, 6XHis antibody, Polyhistidine Tag antibody, HIS6 Tag antibody, 6-His antibody, HHHHHH antibody.
-