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beta-2 Microglobulin antibody

B2M Reactivity: Human, Pig WB, FACS, ELISA, IHC (p), ICC Host: Mouse Monoclonal B2M-02 unconjugated
Catalog No. ABIN93900
  • Target See all beta-2 Microglobulin (B2M) Antibodies
    beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
    Reactivity
    • 204
    • 52
    • 31
    • 27
    • 9
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Pig
    Host
    • 118
    • 110
    • 11
    • 9
    • 1
    • 1
    Mouse
    Clonality
    • 133
    • 116
    • 1
    Monoclonal
    Conjugate
    • 111
    • 33
    • 25
    • 15
    • 13
    • 8
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This beta-2 Microglobulin antibody is un-conjugated
    Application
    • 129
    • 111
    • 73
    • 66
    • 61
    • 42
    • 41
    • 31
    • 27
    • 18
    • 17
    • 15
    • 13
    • 12
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    Specificity
    The mouse monoclonal antibody B2M-02 reacts with beta2-microglobulin (beta2M), an extracellular antigen associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
    Cross-Reactivity (Details)
    Human, Porcine
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Purified isolated human beta2-microglobulin.
    Clone
    B2M-02
    Isotype
    IgG1
    Top Product
    Discover our top product B2M Primary Antibody
  • Application Notes
    Immunohistochemistry (paraffin sections): Heat-mediated antigen retrieval, recommended dilution: 5 μg/mL, positive tissue: liver.
    Western blotting: Recommended dilution: 2 μg/mL, non-reducing conditions preferred.
    Flow cytometry: Recommended dilution: 1 μg/mL, positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes.
    ELISA: Working dilution should be determinated by investigator.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Drbal, Angelisová, Hilgert, Cerný, Novák, Horejsí: "A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells." in: Blood, Vol. 98, Issue 5, pp. 1561-6, (2001) (PubMed).

    Hilgert, Horejsí, Kristofová: "The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin." in: Folia biologica, Vol. 30, Issue 6, pp. 369-76, (1985) (PubMed).

  • Target
    beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
    Alternative Name
    beta2-Microglobulin (B2M Products)
    Synonyms
    Ly-m11 antibody, beta2-m antibody, beta2m antibody, bwm antibody, wu:fa94c05 antibody, wu:fb10a09 antibody, b2m-W01 antibody, b2m-W03 antibody, b2m-Z01 antibody, b2m-Z02 antibody, b2m-Z03 antibody, B2M antibody, b2m antibody, B2MG antibody, wu:fb66f11 antibody, wu:fj01f08 antibody, zgc:64161 antibody, b-2-m antibody, beta-2-microglobulin antibody, beta-2 microglobulin antibody, beta-2-microglobulin S homeolog antibody, beta-2-microglobulin, like antibody, B2M antibody, B2m antibody, b2m antibody, b2m.S antibody, b2ml antibody
    Background
    Beta-2-microglobulin,Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.,B2M
    Gene ID
    567
    UniProt
    P61769
    Pathways
    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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