beta-2 Microglobulin antibody (beta-2-Microglobulin) (HRP)

Details for Product anti-B2M Antibody No. ABIN93906
Antigen
Reactivity
Human
486
100
67
64
16
9
6
4
3
2
2
2
1
1
1
1
1
1
Host
Mouse
322
195
15
11
9
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This beta-2 Microglobulin antibody is conjugated to HRP
50
34
18
17
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
3
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
264
249
183
159
126
95
59
42
40
33
31
24
19
13
8
8
8
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Options
Immunogen Purified human beta2-microglobulin
Clone B2M-01
Isotype IgG2a
Specificity The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
No Cross-Reactivity Mouse (Murine), Cow (Bovine), Dog (Canine), Rabbit, Chicken
Cross-Reactivity (Details) Human
Antigen
Alternative Name beta2-Microglobulin (B2M Antibody Abstract)
Background Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Pathways TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Application Notes The reagent is designed for quantitative determination of soluble beta2-microglobulin/MHC Class I complexes. The reagent can also be used for detection of free beta2-microglobulin by Western blotting.
Comment

The purified antibody is conjugated with horseradish peroxidase (HRP) of high specific activity and RZ=3.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 0.01 % (w/v) thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-beta-2-Microglobulin (B2M) antibody (HRP) (ABIN93906) ELISA analysis of HLA-G and β2-microglobulin in supernatant of HLA-G transfectants wi...
Product cited in: Poláková, Železníková, Russ: "HLA-G5 in the blood of leukemia patients and healthy individuals." in: Leukemia research, Vol. 37, Issue 2, pp. 139-45, 2013 (PubMed).

Khurana, Traum, Aivado, Wells, Guerrero, Grall, Libermann, Schachter: "Urine proteomic profiling of pediatric nephrotic syndrome." in: Pediatric nephrology (Berlin, Germany), Vol. 21, Issue 9, pp. 1257-65, 2006 (PubMed).

Lafon, Prehaud, Megret, Lafage, Mouillot, Roa, Moreau, Rouas-Freiss, Carosella: "Modulation of HLA-G expression in human neural cells after neurotropic viral infections." in: Journal of virology, Vol. 79, Issue 24, pp. 15226-37, 2005 (PubMed).

Hilgert, Horejsí, Kristofová: "The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin." in: Folia biologica, Vol. 30, Issue 6, pp. 369-76, 1985 (PubMed).