beta-2 Microglobulin antibody (beta-2-Microglobulin) (FITC)

Details for Product anti-B2M Antibody No. ABIN93905
Antigen
Reactivity
Human
486
100
67
64
16
9
6
4
3
2
2
2
1
1
1
1
1
1
Host
Mouse
322
195
15
11
9
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This beta-2 Microglobulin antibody is conjugated to FITC
50
33
18
18
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
3
2
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
264
249
183
159
126
95
59
42
40
33
31
24
19
13
8
8
8
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Options
Immunogen Purified human beta2-microglobulin
Clone B2M-01
Isotype IgG2a
Specificity The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
No Cross-Reactivity Mouse (Murine), Cow (Bovine), Dog (Canine), Rabbit, Chicken
Cross-Reactivity (Details) Human
Antigen
Alternative Name beta2-Microglobulin (B2M Antibody Abstract)
Background Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Pathways TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Application Notes The reagent is designed for Flow Cytometry analysis.Suggested working dilution is 1:200. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-beta-2-Microglobulin (B2M) antibody (FITC) (ABIN93905) Confocal microscopy of human β2-microglobulin-transfected canine cells labeled A) wit...
Image no. 2 for anti-beta-2-Microglobulin (B2M) antibody (FITC) (ABIN93905) Western Blotting Western Blotting analysis (non-reducing conditions) of whole cell ly...
Product cited in: Poláková, Železníková, Russ: "HLA-G5 in the blood of leukemia patients and healthy individuals." in: Leukemia research, Vol. 37, Issue 2, pp. 139-45, 2013 (PubMed).

Khurana, Traum, Aivado, Wells, Guerrero, Grall, Libermann, Schachter: "Urine proteomic profiling of pediatric nephrotic syndrome." in: Pediatric nephrology (Berlin, Germany), Vol. 21, Issue 9, pp. 1257-65, 2006 (PubMed).

Lafon, Prehaud, Megret, Lafage, Mouillot, Roa, Moreau, Rouas-Freiss, Carosella: "Modulation of HLA-G expression in human neural cells after neurotropic viral infections." in: Journal of virology, Vol. 79, Issue 24, pp. 15226-37, 2005 (PubMed).

Hilgert, Horejsí, Kristofová: "The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin." in: Folia biologica, Vol. 30, Issue 6, pp. 369-76, 1985 (PubMed).