AKT1 antibody (Internal Region)

Catalog No. ABIN940546

This Goat Polyclonal antibody specifically detects AKT1 in WB, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for AKT1 antibody (Internal Region) (ABIN940546)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AKT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-AKT1 Antibody

Binding Specificity: Internal Region

Purpose: AKT1

Sequence: QDVDQREAPL N

Specificity: All variants represent identical protein (NP_005154.2, NP_001014431.1, NP_001014432.1). This antibody is NOT expected to cross-react with AKT2. It may recognize AKT1 in Rat and Dog.

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QDVDQREAPLN, from the internal region of the protein sequence according to NP_005154.2, NP_001014431.1, NP_001014432.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in MCF7 cell lysates (calculated MW of 55.7 kDa according to NP_005154.2). This molecular weight is routinely observed by other sources. Recommended concentration:0.1-0.3 μg/mL. Primary incubation 1 hour at room tem

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the microtubules/cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: AKT1

Background: AKT1, v-akt murine thymoma viral oncogene homolog 1 , AKT, MGC99656, PKB, PRKBA, RAC, RAC-ALPHA , RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, protein kinase B, rac protein kinase alpha

Gene ID: 207

NCBI Accession: NP_005154, NP_001014431, NP_001014432

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect