UBE2C antibody (Internal Region)

Catalog No. ABIN940571

Product Details anti-UBE2C Antibody

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2C antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: UBE2C / UBCH10

Sequence: SGDKGISAFP ESDN

Specificity: This antibody is expected to recognize isoforms 1 (NP_008950.1), 2 ( NP_861515.1) and 4 (NP_861517.1) . Reported variants NP_861517.1 and NP_861518.1represent identical protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-SGDKGISAFPESDN, from the internal region of the protein sequence according to NP_008950.1, NP_861515.1, NP_861517.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 19 kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 19.7 kDa according to NP_008950.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UBE2C

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Alternative Name: UBE2C (UBE2C Products)

Synonyms: LOC458287 antibody, UBCH10 antibody, dJ447F3.2 antibody, 1110015A16Rik antibody, D2Ertd695e antibody, im:7137135 antibody, zgc:123190 antibody, ubiquitin conjugating enzyme E2 C antibody, ubiquitin-conjugating enzyme E2C antibody, ubiquitin conjugating enzyme E2 C L homeolog antibody, ubiquitin conjugating enzyme E2 C S homeolog antibody, UBE2C antibody, NAEGRDRAFT_82946 antibody, ube2c.L antibody, Ube2c antibody, ube2c.S antibody, ube2c antibody

Background: UBE2C, ubiquitin-conjugating enzyme E2C, UBCH10, dJ447F3.2, OTTHUMP00000031653, OTTHUMP00000031655, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, ubiquitin carrier protein E2-C, ubiquitin-protein ligase C

Gene ID: 11065

NCBI Accession: NP_008950, NP_861515, NP_861517

Pathways: Ubiquitin Proteasome Pathway