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CD31 antibody (Platelet/endothelial Cell Adhesion Molecule) (FITC) Primary Antibody

PECAM1 Reactivity: Human FACS, WB Host: Mouse Monoclonal MEM-05 FITC
Pubmed (1)
Catalog No. ABIN94071
$455.40
Plus shipping costs $45.00
100 tests
local_shipping Shipping to: United States
Delivery in 5 to 7 Business Days
  • Target
    CD31 (PECAM1)
    Reactivity
    Human
    • 368
    • 161
    • 96
    • 56
    • 55
    • 19
    • 18
    • 15
    • 9
    • 8
    • 6
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Host
    Mouse
    • 248
    • 170
    • 56
    • 6
    • 3
    • 1
    • 1
    Clonality
    Monoclonal
    Conjugate
    This CD31 antibody is conjugated to FITC
    • 194
    • 45
    • 37
    • 35
    • 19
    • 12
    • 10
    • 9
    • 8
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Application
    Flow Cytometry (FACS), Western Blotting (WB)
    • 320
    • 210
    • 171
    • 134
    • 72
    • 54
    • 54
    • 42
    • 29
    • 27
    • 19
    • 18
    • 15
    • 14
    • 7
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Specificity
    The antibody MEM-05 reacts with CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.
    Cross-Reactivity (Details)
    Human
    Immunogen
    Leukocytes of patient suffering from LGL-type leukaemia
    Clone
    MEM-05
    Isotype
    IgG1
  • Application Notes
    The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
    Comment

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

    Restrictions
    For Research Use only
  • Reconstitution
    No reconstitution is necessary.
    Buffer
    The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
  • Prager, Staffler, Majdic, Säemann, Godár, Zlabinger, Stockinger: "Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 4, pp. 2364-71, 2001 (PubMed).

  • Target
    CD31 (PECAM1)
    Alternative Name
    CD31 (PECAM1 Antibody Abstract)
    Synonyms
    CD31, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, endoCAM, C85791, Cd31, Pecam, platelet/endothelial cell adhesion molecule 1, platelet and endothelial cell adhesion molecule 1, pecam1, PECAM1, Pecam1
    Background
    CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
    Pathways
    Regulation of Actin Filament Polymerization
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