CD44 antibody
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- Target See all CD44 Antibodies
- CD44
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Reactivity
- Human, Dog, Pig
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD44 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- Specificity
- The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.), it is negative on platelets and hepatocytes.
- Cross-Reactivity (Details)
- Human, Porcine, Canine (Dog)
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- COS-7 cells (African Green Monkey).
- Clone
- MEM-263
- Isotype
- IgG1
- Top Product
- Discover our top product CD44 Primary Antibody
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- Application Notes
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Flow cytometry: Recommended dilution: 4 μg/mL.
Western blotting: Recommended dilution: 2 μg/mL, 60 min on vertical incubator, positive control: Kg-1a human acute leukemia cell lysate, JURKAT human leukemia T-cell lysate, non-reducing conditions, 6 % separating gel.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: uterus, myometrium. - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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Characterisation of stemness and multipotency of ovine muscle-derived stem cells from various muscle sources." in: Journal of anatomy, Vol. 239, Issue 2, pp. 336-350, (2021) (PubMed).
: "Vascular wall-resident CD44+ multipotent stem cells give rise to pericytes and smooth muscle cells and contribute to new vessel maturation." in: PLoS ONE, Vol. 6, Issue 5, pp. e20540, (2011) (PubMed).
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Characterisation of stemness and multipotency of ovine muscle-derived stem cells from various muscle sources." in: Journal of anatomy, Vol. 239, Issue 2, pp. 336-350, (2021) (PubMed).
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- Target
- CD44
- Alternative Name
- CD44 (CD44 Products)
- Synonyms
- CDW44 antibody, CSPG8 antibody, ECMR-III antibody, HCELL antibody, HUTCH-I antibody, IN antibody, LHR antibody, MC56 antibody, MDU2 antibody, MDU3 antibody, MIC4 antibody, Pgp1 antibody, AU023126 antibody, AW121933 antibody, AW146109 antibody, HERMES antibody, Ly-24 antibody, Pgp-1 antibody, CD44A antibody, METAA antibody, RHAMM antibody, XCD44 antibody, cd44 antibody, cdw44 antibody, cspg8 antibody, ecmr-iii antibody, hcell antibody, lhr antibody, mc56 antibody, mdu2 antibody, mdu3 antibody, mic4 antibody, mutch-i antibody, pgp1 antibody, CD44 molecule (Indian blood group) antibody, syndecan 4 antibody, CD44 antigen antibody, CD44 molecule (Indian blood group) S homeolog antibody, CD44 antibody, SDC4 antibody, Cd44 antibody, cd44.S antibody
- Background
- CD44 Molecule,CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.,PGP-I, HUTCH-I, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, MC56, MIC4, INLU, LHR
- Gene ID
- 960
- UniProt
- P16070
- Pathways
- Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
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