MICA antibody

Catalog No. ABIN94335

The Mouse Monoclonal anti-MICA antibody (Clone MEM-123) (ABIN94335) specifically detects MICA in ELISA, FACS and IP. The antibody is reactive with Human, Cow and Non-Human Primate samples.

Quick Overview for MICA antibody (ABIN94335)

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Reactivity: Human, Cow, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This MICA antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: MEM-123

Product Details anti-MICA Antibody

Purpose: Anti-HLA Class I Purified

Cross-Reactivity (Details): Human, Non-Human Primates, Bovine

Characteristics: The antibody MEM-123 reacts with an extracellular epitope of all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. The antibody MEM-123 completely blocks binding of classical W6/32 to surface-expressed HLA-G, but does not cross-blocks the antibody MEM-G/9.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: COS-7 African green monkey kidney cells

Isotype: IgG3

Application Details

Application Notes: Flow cytometry: Recommended dilution: 4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for MICA

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Alternative Name: HLA Class I

Background: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Gene ID: 35

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response