MICA antibody (MHC Class I Polypeptide-Related Sequence A)

Details for Product anti-MICA Antibody No. ABIN94335
Antigen
Reactivity
Human, Non-Human Primate, Cow (Bovine)
142
20
15
14
14
6
4
1
Host
Mouse
87
53
Clonality (Clone)
Monoclonal ()
Conjugate
This MICA antibody is un-conjugated
11
7
6
2
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA
101
61
51
37
32
16
14
11
11
9
7
6
5
5
1
1
1
1
Options
Immunogen COS-7 African green monkey kidney cells
Clone MEM-123
Isotype IgG3
Specificity The antibody MEM-123 reacts with all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types.
The antibody MEM-123 completely blocks binding of classical W6/32 to surface-expressed HLA-G, but does not cross-blocks the antibody MEM-G/9.
Cross-Reactivity (Details) Human, Non-Human Primates, Bovine
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name HLA-Class I (MICA Antibody Abstract)
Background HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Pathways Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.