Insulin antibody

Catalog No. ABIN94408

This Mouse Monoclonal antibody specifically detects Insulin in ELISA, IHC (p), ICC and RIA. It exhibits reactivity toward Human, Pig and Cow and has been mentioned in 2+ publications.

Quick Overview for Insulin antibody (ABIN94408)

Target: Insulin (INS)

Reactivity: Human, Pig, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Radioimmunoassay (RIA)

Clone: IN-05

Product Details anti-Insulin Antibody

Purpose: Anti-Insulin Purified

Specificity: The antibody IN-05 reacts with insulin, one of the major regulatory endocrine hormones of intermediate metabolism, normally secreted by the beta cells (a type of islet cells) of the pancreas, it is also present in tumors of B cell origin such as insulinoma.

Cross-Reactivity (Details): Human, Bovine, Porcine

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Porcine insulin.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Insulin antibody (ABIN94408)

Meesaragandla, Karanth, Janke, Delcea: "Biopolymer-coated gold nanoparticles inhibit human insulin amyloid fibrillation." in: Scientific reports, Vol. 10, Issue 1, pp. 7862, (2020) (PubMed).

Horejsí, Hilgert, Kristofová, Satayalai: "Murine hybridoma monoclonal antibodies against insulin: cross-reactivity with insulins of three species and blocking of insulin binding to its receptor." in: Immunology letters, Vol. 8, Issue 5, pp. 279-83, (1985) (PubMed).

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Proinsulin,Insulin and glucagon are pancreatic endocrine hormones secreted by islet cells within the pancreas. The stimulus for insulin secretion is a HIGH blood glucose. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population.,IDDM, INS

Gene ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation