Insulin antibody (INS)

Details for Product anti-INS Antibody No. ABIN94408
Antigen
Reactivity
Human, Pig (Porcine), Cow (Bovine)
684
260
226
207
183
104
10
9
6
6
5
4
3
2
2
2
2
2
1
1
1
1
1
1
Host
Mouse
520
201
46
4
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Insulin antibody is un-conjugated
39
24
24
10
8
8
8
8
8
8
8
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), ELISA
331
227
196
154
143
113
81
66
61
35
28
28
26
25
20
13
5
5
4
4
4
3
2
2
2
1
1
1
1
1
Options
Immunogen Porcine insulin.
Clone IN-05
Isotype IgG1
Specificity The antibody IN-05 reacts with insulin, one of the major regulatory endocrine hormones of intermediate metabolism, normally secreted by the beta cells (a type of islet cells) of the pancreas, it is also present in tumors of B cell origin such as insulinoma.
Cross-Reactivity (Details) Human, Bovine, Porcine
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Insulin and glucagon are pancreatic endocrine hormones secreted by islet cells within the pancreas. The stimulus for insulin secretion is a HIGH blood glucose. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population.
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Meesaragandla, Karanth, Janke, Delcea: "Biopolymer-coated gold nanoparticles inhibit human insulin amyloid fibrillation." in: Scientific reports, Vol. 10, Issue 1, pp. 7862, 2020 (PubMed).

Horejsí, Hilgert, Kristofová, Satayalai: "Murine hybridoma monoclonal antibodies against insulin: cross-reactivity with insulins of three species and blocking of insulin binding to its receptor." in: Immunology letters, Vol. 8, Issue 5, pp. 279-83, 1985 (PubMed).