T Cell Receptor Associated Transmembrane Adaptor 1 (TRAT1) (AA 29-186) antibody

Details for Product No. ABIN94489
Antigen
Epitope
AA 29-186
15
10
8
7
7
2
2
Reactivity
Human
49
16
3
Host
Mouse
30
17
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
8
3
3
3
2
2
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
41
25
16
12
1
1
1
Options
Immunogen Recombinant intracellular domain (aa 29-186) of human TRIM.
Clone TRIM-04
Isotype IgG2a
Specificity The antibody TRIM-04 reacts with T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
No Cross-Reactivity Mouse (Murine)
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name TRIM (TRAT1 Antibody Abstract)
Background TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-T Cell Receptor Associated Transmembrane Adaptor 1 (TRAT1) (AA 29-186) antibody (ABIN94489) Western blot of human Jurkat T cell line
Product cited in: Kirchgessner, Dietrich, Scherer, Isomäki, Korinek, Hilgert, Bruyns, Leo, Cope, Schraven: "The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain." in: The Journal of experimental medicine, Vol. 193, Issue 11, pp. 1269-84, 2001 (PubMed).