AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (AA 455-468)

Details for Product anti-AKT2 Antibody No. ABIN94790
Antigen
Epitope
AA 455-468
73
57
28
24
21
20
15
11
9
9
8
7
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
437
209
172
56
55
51
50
49
46
45
45
45
28
18
4
3
2
1
1
Host
Rabbit
355
78
13
7
3
2
Clonality
Polyclonal
Conjugate
This AKT2 antibody is un-conjugated
25
24
21
19
19
17
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
383
265
171
85
64
44
38
27
26
21
19
12
8
6
4
3
2
1
Options
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 455-468 (of 481) of human AKT2 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Antigen
Alternative Name AKT2 (AKT2 Antibody Abstract)
Background AKT2 is also known as V-AKT Murine Thymoma Viral Oncogene Homolog 2 antibody, Rac protein kinase beta antibody, PKB beta antibody or PRKBB antibody.  AKT2 is an isoform of the phosphoinositide-dependent serine-threonine protein kinase AKT and is enriched in insulin-responsive tissues and has been implicated in the metabolic actions of the hormone.  AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Over-expression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.  AKT2 mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin).  PI 3-kinase recruits AKT2 to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT2 dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.  AKT2 has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Synonyms: AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta
Gene ID 208, 164731
UniProt P31751
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
Application Notes This antibody was tested by ELISA and immunoblotting and was found to be reactive with both unphosphorylated and phosphorylated AKT2 in a lysate of HEK293 cells.  Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.This product has been assayed by immunoblot against a HEK293 cell lysate and is reactive at a 1:1,000 dilution showing a band at approximately 60 kDa.   A working dilution of 1:4,000 to 1:16,000 is suggested for this product in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature against 0.1 ug of the immunizing peptide. Researchers should determine optimal titers for other applications.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 455-468) antibody (ABIN94790) Western Blot of Rabbit Anti-AKT2 antibody. Lane 1: 0 min. Lane 2: 15 min 4OHT (tamoxi...
Image no. 2 for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 455-468) antibody (ABIN94790) Western Blot of Rabbit anti-AKT2 antibody. Lane 1: mouse skeletal muscle tissue lysat...
Image no. 3 for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 455-468) antibody (ABIN94790) Western Blot of Rabbit anti-AKT2 antibody. Lane 1: HEK293 lysate Lane 2: HEK293 lysat...
Product cited in: Subramanyam, Takahashi, Hasegawa, Mohri, Okada: "Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase." in: The Journal of biological chemistry, Vol. 285, Issue 9, pp. 6109-17, 2010 (PubMed).