ALT antibody

Catalog No. ABIN94799

Product Details anti-ALT Antibody

Target: ALT (Alanine Aminotransferase (ALT))

Reactivity: Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: This ALT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Alanine Transaminase from other sources is unknown.

Purity: Anti-ALANINE TRANSAMINASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Alanine Transaminase [Pig Heart].

Endotoxin Level: Low Endotoxin : No

Immunogen: Alanine Transaminase (ALT) [Pig Heart]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: ALANINE TRANSAMINASE antibody has been assayed against 1.0 µg of Alanine Transaminase [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2.000 to 1:8.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for ALT

Target: ALT (Alanine Aminotransferase (ALT))

Alternative Name: Alanine Transaminase (ALT Products)

Synonyms: AAT1 antibody, ALT1 antibody, GPT1 antibody, GPT antibody, Gpt1 antibody, An11g02620 antibody, AO090003000164 antibody, 233.t00009 antibody, 24.t00016 antibody, 1300007J06Rik antibody, 2310022B03Rik antibody, ALT antibody, Gpt-1 antibody, AU021132 antibody, Dpagt2 antibody, Gnpta antibody, gpt antibody, ALANINE AMINOTRANSFERAS antibody, AtAlaAT1 antibody, AtAlaATc antibody, T13M22.3 antibody, T13M22_3 antibody, alanine aminotransferas antibody, glutamic--pyruvic transaminase antibody, glutamic-pyruvate transaminase (alanine aminotransferase) antibody, alanine aminotransferase antibody, alanine aminotransferase 1 antibody, glutamic pyruvic transaminase, soluble antibody, dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) antibody, alanine aminotransferase 2-like antibody, Alanine aminotransferase 1, mitochondrial antibody, GPT antibody, Gpt antibody, Tb927.1.3950 antibody, ANI_1_368094 antibody, AOR_1_284154 antibody, ALAT_1 antibody, ALAAT1 antibody, EHI_159710 antibody, EHI_096750 antibody, LOC5572541 antibody, ALAT antibody, AAT2 antibody, Dpagt1 antibody, LOC100537633 antibody, AlaAT1 antibody

Background: Synonyms: AAT1 antibody, Alanine aminotransferase 1 antibody, alanine aminotransferase antibody, ALT 1 antibody, ALT1 antibody, Glutamic alanine transaminase 1 antibody

Gene ID: 2875, 113565

UniProt: P13191