TEK antibody (AA 19-745)

Catalog No. ABIN951222

The Rabbit Polyclonal anti-TEK antibody is suitable to detect TEK in samples from Human and Mouse. It has been validated for WB.

Quick Overview for TEK antibody (AA 19-745) (ABIN951222)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TEK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TEK Antibody

Binding Specificity: AA 19-745

Specificity: Recognizes TIE-2 (CD202b/TEK)

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Protein A Chromatography

Immunogen: Highly pure (>95%) recombinant Human soluble TIE-2 (Thr19-Lys745) derived from Insect cells.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at RT for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C. Avoid repeated freeze-thaw cycles!

Expiry Date: 6 months

Target Details for TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: CD202b / TEK

Background: TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/TEK comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind TIE-2 with high affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or TIE-2 display similar angiogenic defects.Synonyms: Angiopoietin-1 receptor, TIE-2, TIE2, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK

Gene ID: 7010

NCBI Accession: NP_000450

Pathways: RTK Signaling, Growth Factor Binding