TEK antibody (AA 23-737)

Catalog No. ABIN951225

The Rabbit Polyclonal anti-TEK antibody is suitable to detect TEK in samples from Mouse. It has been validated for WB.

Quick Overview for TEK antibody (AA 23-737) (ABIN951225)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TEK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TEK Antibody

Binding Specificity: AA 23-737

Specificity: Recognizes TIE-2 (CD202b/TEK)

Cross-Reactivity (Details): Species reactivity (tested):Mouse, Human (Weak).

Purification: Protein A Chromatography

Immunogen: Highly pure (>95%) recombinant Mouse soluble TIE-2 (Ala23-Ala737) derived from Insect cells.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at RT for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C. Avoid repeated freeze-thaw cycles!

Expiry Date: 6 months

Target Details for TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: CD202b / TEK

Background: Recombinant Mouse soluble TIE-1 was fused with a 6x His-tag at the C-terminus. The soluble receptor protein consists of the full extracellular domain (Ser22-Ala748). Mouse sTIE-1 monomer has a calculated molecular mass of approximately 79,8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 95 kDa protein in SDS-PAGE under reducing conditions. TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.Synonyms: Angiopoietin-1 receptor, TIE-2, TIE2, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK

Gene ID: 7010

NCBI Accession: NP_000450

Pathways: RTK Signaling, Growth Factor Binding