Anti-human Glycophorin A (GpA) Polyclonal Antibody targets the human Glycophorin A protein. This FITC conjugated polyclonal antibody recognizes various epitopes on the GpA protein. The 23 residue hydrophobic transmembrane domain has been shown to mediate non-covalent dimerization of the protein under conditions of SDS page (and in other detergents). We also sell an ascites purified version of this antibody (AP31671PU-N).
Purification
Affinity chromatography on Protein G
Immunogen
Human Glycophorin A transmembrane protein Donor: Rabbit serum
GYPA
Reactivity: Human
WB, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Concentration
0,1 mg/mL
Buffer
PBS containing 0.02 % sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
This 131 amino acid, 36 KDa, transmembrane sialoglycoprotein spans the membrane of erythrocytes once. GpA presents its N terminus at the extracellular surface of the human red blood cell, has a single transmembrane domain and an internal cytoplasmic C terminus. GpA contributes to the expression of blood group antigen M and N specificities, which work to prevent red blood cell aggregation in circulation. Studies show that GpA is expressed early on the cell surface during erythropoiesis as pluripotent stem cells are recruited to differentiate into erythrocytes. This makes GpA a useful marker for studying the developmental biology of hematopoietic progenitor cells.Synonyms: GPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha