Mitotic Cells antibody

Catalog No. ABIN959451

Product Details

Target: Mitotic Cells

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Specificity: Strongly stains mitotic cells.

Purification: Purified

Immunogen: Total cell lysate of the human bladder carcinoma cell line T24

Clone: 8B3G

Isotype: IgM

Application Details

Application Notes: Approved: Flo (1:50 - 1:100), ICC (1:50 - 1:100), IHC

Usage: Strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) antibody clearly distinguishes these M-phase cells from cell at other stages of the cell cycle. Dynamic information can be obtained by combining BrdU incorporation with antibody staining, which can distinguish and quantitate the four major fractions of the cell cycle.

Not recommended for: IHC-P, WB

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details

Target: Mitotic Cells