Annexin V antibody

Catalog No. ABIN959502

Product Details anti-Annexin V Antibody

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Annexin V antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Specifically reacts with annexin A5, directed to the phospholipid binding domain of annexin A5, and as such it inhibits annexin A5 binding to anionic phospholipid membranes, phospholipid-containing vesicles, activated platelets and apoptotic cells for more than 95 % . Does not remove annexin A5 bound to phospholipid surfaces, indicating that the epitope is not accessible after interaction with annexin A5. It shows no cross reactivity with Annexins I, II, III, IV, VI, VII and VIII in ELISA or in immunoprecipitation. It does not recognize monkey and rat Annexin V in ELISA.

No Cross-Reactivity: Monkey, Rat (Rattus)

Purification: Purified

Immunogen: Recombinant human annexin A5

Type of Immunogen: Recombinant protein

Clone: RUU-WAC2A

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-Fr (1:50 - 1:100), WB (1:100 - 1:500)

Usage: For immuno-histochemistry of paraffin sections, a pretreatment step with 2 % amino-propyl-triethoxysilan (in 100 % ethanol) and 0.2 % BSA is recommended. The sections are heated for 15 minutes at 100 °C in a citrate buffer (10 mM trisodium citrate,  pH 6,0).

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for Annexin V

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Alternative Name: ANXA5 / Annexin V (ANXA5 Products)

Synonyms: anx antibody, anx5 antibody, ANX V antibody, anxa5 antibody, cb989 antibody, wu:fa98f06 antibody, wu:fj10f10 antibody, MGC89158 antibody, ANX5 antibody, ENX2 antibody, PP4 antibody, RPRGL3 antibody, Anx5 antibody, R74653 antibody, LC5 antibody, enx2 antibody, annexin A5 antibody, annexin A5b antibody, Annexin A5 antibody, annexin A5 L homeolog antibody, ANXA5 antibody, anxa5b antibody, anxa5 antibody, Anxa5 antibody, anxa5.L antibody

Background: Name/Gene ID: ANXA5

Synonyms: ANXA5, ANX5, Anchorin CII, Calphobindin I, CBP-I, ENX2, PP4, RPRGL3, Lipocortin V, Thromboplastin inhibitor, VAC-alpha, Vascular anticoagulant-alpha, PAP-I, Annexin A5, Annexin V, Annexin-5, Endonexin II

Gene ID: 308

UniProt: P08758

Pathways: Apoptosis