Fluorescein antibody (Biotin)

Catalog No. ABIN964654

Supplier Product No.: 700-106-096

This Goat Polyclonal antibody specifically detects Fluorescein in WB, ELISA and IHC.

Quick Overview for Fluorescein antibody (Biotin) (ABIN964654)

Target: Fluorescein

Fragment: F(ab')2 fragment

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details

Supplier Product No.: 700-106-096

Supplier: Rockland

Purpose: F(Ab')2 Fluorescein Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum and Fluorescein conjugated BSA.

Characteristics: Synonyms: goat F(ab')2 anti-Fluorescein Antibody biotin Conjugation, goat F(ab')2 anti-Fluorescein biotin Conjugated Antibody, goat F(ab')2 anti-FITC Antibody biotin Conjugation

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen:

Immunogen: Fluorescein conjugated to Goat IgG

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: F(ab')2 Anti-Fluorescein Antibody Conjugated to biotin has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: Fluorescein

Target Type: Chemical

Background: Background: F(ab')2 Anti-Fluorescein Antibody generated in goat detects fluorescein. It is an organic dye used as a fluorescent tracer in many application including cellular staining. This F(ab')2 Anti-Fluorescein Antibody is Biotin conjugated.