Fluorescein antibody (Biotin)
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- Target
- Fluorescein
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
- Supplier Product No.
- 600-106-096
- Supplier
- Rockland
- Purpose
- Fluorescein Antibody Biotin Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum and Fluorescein conjugated IgG.
- Characteristics
- Synonyms: goat Anti-FITC antibody biotin conjugation, biotin conjugated goat anti-fluorescein antibody, biotin conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody biotin conjugation
- Purification
- Fluorescein Antibody Biotin Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Immunogen
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Immunogen: Fluorescein conjugated to Goat IgG
Immunogen Type: Other
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Fluorescein Antibody Biotin Conjugated has been tested by ELISA, dot blot and western blot and is suitable for immunoblotting, ELISA, in situ hybridization, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Fluorescein
- Target Type
- Chemical
- Background
- Background: Fluorescein Antibody Biotin Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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