ABO, Blood Group A Antigen antibody

Catalog No. ABIN965677

Product Details

Target: ABO, Blood Group A Antigen

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Specificity: This antibody reacts with blood group substance A, subtype A1 and A2, on the cell membranes of epithelium, endothelium and red blood cells.

Purification: Concentrated.

Immunogen: Synthetic trisaccharide, alpha-D-N-Acetyl-galactosamine-(1-3)-(alpha-L-Fuc(1-2))-beta-D-Gal.

Clone: B45-1

Isotype: IgM

Application Details

Application Notes: Immunohistochemistry: 1:50.
Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.

Comment:

Recommended Positive Control: Human Tissues

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References

Manimala, Roach, Li, Gildersleeve: "High-throughput carbohydrate microarray profiling of 27 antibodies demonstrates widespread specificity problems." in: Glycobiology, Vol. 17, Issue 8, pp. 17C-23C, (2007) (PubMed).

Chapman, Allhoff, Proppe, Prout: "Use of monoclonal antibodies for the localization of tissue isoantigens A and B in transitional cell carcinoma of the upper urinary tract." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 31, Issue 4, pp. 557-61, (1983) (PubMed).

Target Details

Target: ABO, Blood Group A Antigen

Alternative Name: Blood Group Antigen A