ABO, Blood Group A Antigen antibody
-
- Target
- ABO, Blood Group A Antigen
- Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Un-conjugated
-
Application
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
- Specificity
- This antibody reacts with blood group substance A, subtype A1 and A2, on the cell membranes of epithelium, endothelium and red blood cells.
- Purification
- Concentrated.
- Immunogen
- Synthetic trisaccharide, alpha-D-N-Acetyl-galactosamine-(1-3)-(alpha-L-Fuc(1-2))-beta-D-Gal.
- Clone
- B45-1
- Isotype
- IgM
-
-
- Application Notes
-
Immunohistochemistry: 1:50.
Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory. - Comment
-
Recommended Positive Control: Human Tissues
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.2 mg/ml.
- Buffer
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
-
-
High-throughput carbohydrate microarray profiling of 27 antibodies demonstrates widespread specificity problems." in: Glycobiology, Vol. 17, Issue 8, pp. 17C-23C, (2007) (PubMed).
: "Use of monoclonal antibodies for the localization of tissue isoantigens A and B in transitional cell carcinoma of the upper urinary tract." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 31, Issue 4, pp. 557-61, (1983) (PubMed).
: "
-
High-throughput carbohydrate microarray profiling of 27 antibodies demonstrates widespread specificity problems." in: Glycobiology, Vol. 17, Issue 8, pp. 17C-23C, (2007) (PubMed).
-
- Target
- ABO, Blood Group A Antigen
- Alternative Name
- Blood Group Antigen A
-