ABO (Blood Group Antigen B) antibody

Catalog No. ABIN965678

Product Details

Target: ABO (Blood Group Antigen B)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody stains erythrocytes and vascular epthelium of blood group B controls.

Purification: Concentrated.

Immunogen: Blood Group B erythrocytes.

Clone: B460

Isotype: IgM

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100.
Staining Procedure: This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using on formalin-fixed paraffin embedded tissues a antigen demasking procedure is recommended.The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Comment:

Recommended Positive Control: Human Tissue (Blood Group B or AB)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References

Ricard, Boboyono, Postel, Collet, Canarelli: "[Gastric rupture in children. Apropos of a case]." in: Annales de pédiatrie, Vol. 36, Issue 7, pp. 479-81, (1989) (PubMed).

Chapman, Allhoff, Proppe, Prout: "Use of monoclonal antibodies for the localization of tissue isoantigens A and B in transitional cell carcinoma of the upper urinary tract." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 31, Issue 4, pp. 557-61, (1983) (PubMed).

Bundle, Gidney, Kassam, Rahman: "Hybridomas specific for carbohydrates; synthetic human blood group antigens for the production, selection, and characterization of monoclonal typing reagents." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 129, Issue 2, pp. 678-2, (1982) (PubMed).

Target Details

Target: ABO (Blood Group Antigen B)

Alternative Name: Blood Group Antigen B

Gene ID: 4059