TNNC1 antibody
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- Target See all TNNC1 Antibodies
- TNNC1 (Cardiac Troponin C (TNNC1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TNNC1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- Specificity
- This antibody is specific for human cardiac troponin subunit I, a 21 kD protein responsible for inhibiting actomyosin Mg2+-ATPase activity. This antibody has less than 0.1% reactivity with human skeletal TNI as measured by ELISA.
- Characteristics
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High temp. antigen demasking for FFPE tissue sections. Deparaffinize and rehydrate tissue sections.
10mM citrate buffer: pH 6.0. Boil 10-20 mins, followed by cooling 10-20 mins
OR Process tissue sections in Pressure cooker for 5 mins in citrate buffer followed by cooling 10-20 mins to RT. Wash tissue sections with buffer (PBS or Automation buffer,) - Purification
- Concentrated.
- Immunogen
- Purified human cardiac troponin I.
- Clone
- 2D5
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product TNNC1 Primary Antibody
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- Application Notes
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Immunohistochemistry:1:25. For formalin-fixed paraffin-embedded (FFPE) tissue sections antigen demasker (10 mM citrate buffer pH 6.0 ) is required. Boil the tissue 10-20 mins in this buffer, followed by cooling to RT.
Western blotting: 1:50-1:100.
ELISA: 1:500-1:1000
Immunoprecipitation.
Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:25-1:50 in IHC. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results. This product can also be used on frozen tissue sections. The optimal conditions should be determined by the individual laboratory. - Comment
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Cellular Localization: cytoplasmic staining of cardiac muscle cells.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.4 mg/ml.
- Buffer
- 20 mM tris-borate, 150 mM Sodium Chloride, BMC-6 carrier polysaccharides, pH 7.5
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- TNNC1 (Cardiac Troponin C (TNNC1))
- Alternative Name
- Cardiac Troponin-1 (TNNC1 Products)
- Synonyms
- CMD1Z antibody, CMH13 antibody, TN-C antibody, TNC antibody, TNNC antibody, cTnC antibody, tnnc1 antibody, zgc:103465 antibody, PCTNC1 antibody, cmd1z antibody, cmh13 antibody, tnc antibody, tnnc antibody, MGC80066 antibody, TNNC1 antibody, AI874626 antibody, TnC antibody, cTnI antibody, tncc antibody, Tncc antibody, stnnc antibody, zgc:86932 antibody, troponin C1, slow skeletal and cardiac type antibody, troponin C type 1a (slow) antibody, troponin C type 1 (slow) L homeolog antibody, cardiac troponin C antibody, troponin C, cardiac/slow skeletal antibody, troponin C type 1b (slow) antibody, TNNC1 antibody, tnnc1a antibody, tnnc1.L antibody, tnnc1 antibody, Tnnc1 antibody, tnnc1b antibody
- Molecular Weight
- 21 kDa
- Gene ID
- 7134
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