CD13 Myeloid Cell antibody

Catalog No. ABIN965764

Product Details

Target: CD13 Myeloid Cell

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts with CD13, a 150 kD cell surface glycoprotein. The CD13 antigen is identical to aminopeptidase N. The specificity of these antibodies are similar to those of WM-15 and My7.

Purification: Purified.

Immunogen: Human KG-1 (myeloid) cultured cells.

Clone: B347 (B-F10)

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections.

Comment:

Recommended Positive Control: Tonsil and Lymph Node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References

Look, Ashmun, Shapiro, Peiper: "Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N." in: The Journal of clinical investigation, Vol. 83, Issue 4, pp. 1299-307, (1989) (PubMed).

Sakai, Hattori, Sagawa, Yokoyama, Takatsuki: "Biochemical and functional characterization of MCS-2 antigen (CD13) on myeloid leukemic cells and polymorphonuclear leukocytes." in: Cancer research, Vol. 47, Issue 21, pp. 5572-6, (1987) (PubMed).

Look, Peiper, Rebentisch, Ashmun, Roussel, Rettenmier, Sherr: "Transfer and expression of the gene encoding a human myeloid membrane antigen (gp150)." in: The Journal of clinical investigation, Vol. 75, Issue 2, pp. 569-79, (1985) (PubMed).

Target Details

Target: CD13 Myeloid Cell

Alternative Name: CD13,Myeloid Cell

Gene ID: 290