CD20 (B Cells) (Extracellular Domain) antibody
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- Target
- CD20 (B Cells)
- Binding Specificity
- Extracellular Domain
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody reacts with an antigen present on most of the B-cells. They react with a 33 kD polypeptide and a minor component of a 30 kD polypeptide present in B-cells.
- Purification
- Purified.
- Immunogen
- Lymphoblastoid cell line DAUDI.
- Clone
- B342 (B9E9)
- Isotype
- IgG2a
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- Application Notes
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Immunofluorescence: 5-20 ug/mL
Flow cytometry: 1-5 ug/10^6 cells
Western blotting: 1-5 ug/mL.
Immunohistochemistry: 5-20 mg/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections. - Comment
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Cellular Localization: Cell membrane.
Recommended Positive Control: Tonsil and Lymph Node - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/ml.
- Buffer
- 50 mM Sodium phosphate, 150 mM Sodium Chloride, BSA 0.2%, pH 8.0
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells." in: The Journal of biological chemistry, Vol. 270, Issue 22, pp. 13099-104, (1995) (PubMed).
: "Transfection of the CD20 cell surface molecule into ectopic cell types generates a Ca2+ conductance found constitutively in B lymphocytes." in: The Journal of cell biology, Vol. 121, Issue 5, pp. 1121-32, (1993) (PubMed).
: "
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Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells." in: The Journal of biological chemistry, Vol. 270, Issue 22, pp. 13099-104, (1995) (PubMed).
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- Target
- CD20 (B Cells)
- Molecular Weight
- 33-37 kDa
- Gene ID
- 931
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