CD74, MHC CLASS11 INVARIANT CHAIN antibody
Reactivity: Human
IHC (fro), FACS, IF, WB
Host: Mouse
Monoclonal
B318 (LN-2)
unconjugated
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- Target
- CD74, MHC Class 11 invariant Chain
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
- Specificity
- This antibody reacts with several isoforms of the CD74 antigen, the invariant chain of HLA-DR.
- Purification
- Purified.
- Immunogen
- Cell extracts from diffuse histolytic lymphoma SU-DHL-4 cells.
- Clone
- B318 (LN-2)
- Isotype
- IgG1
-
- Application Notes
-
Immunofluorescence: 10-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunoblotting: 1-5 ug/mL.
Immunohistochemistry: 10-20 ug/mL on frozen or formalin fixed paraffin embedded tissue sections using avidin-biotin system.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 10-20 ug/mL in IHC. The optimal conditions should be determined by the individual laboratory. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique (see instructions below) is strongly recommended for consistent and reproducible results This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 10-20 ug/mL in IHC. The optimal conditions should be determined by the individual laboratory. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results - Comment
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Recommended Positive Control: Tonsil
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.4 mg/ml.
- Buffer
- 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
-
- Target
- CD74, MHC Class 11 invariant Chain
- Alternative Name
- CD74,MHC Class11 Invariant Chain
- Gene ID
- 972
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