GRK2 antibody

Catalog No. ABIN966226

This Mouse Monoclonal antibody specifically detects GRK2 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse, Rat and Monkey.It has been mentioned in 1 publication

Quick Overview for GRK2 antibody (ABIN966226)

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 3F8

Product Details anti-GRK2 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human GRK2 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-GRK2 antibody (ABIN966226)

Chen, Long, Wu, Jiang, Ma: "EGF transregulates opioid receptors through EGFR-mediated GRK2 phosphorylation and activation." in: Molecular biology of the cell, Vol. 19, Issue 7, pp. 2973-83, (2008) (PubMed).

Target Details for GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Alternative Name: GRK2

Background: The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of betaadrenergic receptor to G protein is involved in the pathogenesis of the failing heart.
Tissue specificity: Expressed in peripheral blood leukocytes
Synonyms: GRK2, BARK1, FLJ16718, BETA-ARK1, ADRBK1

Molecular Weight: 80kDa

Gene ID: 156

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling