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p53 antibody

Cited in 3+ publications. The Rabbit Polyclonal anti-p53 antibody (ABIN966759) specifically detects p53 in IHC. The antibody is reactive with Human samples.
Catalog No. ABIN966759
$411.13
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 4 to 7 Business Days

Quick Overview for p53 antibody (ABIN966759)

Target

See all p53 (TP53) Antibodies
p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity

  • 1053
  • 341
  • 270
  • 140
  • 75
  • 74
  • 54
  • 37
  • 11
  • 11
  • 10
  • 7
  • 6
  • 3
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  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Human

Host

  • 788
  • 293
  • 3
  • 3
  • 2
  • 2
Rabbit

Clonality

  • 578
  • 512
  • 1
Polyclonal

Conjugate

  • 555
  • 55
  • 31
  • 23
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  • 13
  • 12
  • 11
  • 11
  • 11
  • 11
  • 11
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
This p53 antibody is un-conjugated

Application

  • 823
  • 343
  • 293
  • 284
  • 254
  • 171
  • 149
  • 149
  • 131
  • 122
  • 64
  • 57
  • 25
  • 15
  • 12
  • 9
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  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
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  • 1
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (IHC)
  • Specificity

    P53 (Ab-315) antibody detects endogenous levels of total p53 protein.

    Purification

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    Immunogen

    The antiserum was produced against synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of serine 315 (S-S-SP-P-Q).
  • Application Notes

    WB: 1:500~1:1000.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    100ug/100ul.

    Buffer

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C
  • Merrick, Zhou, Martin, Jeyarajah, Parker, Selkirk, Tomer, Borchers: "Site-specific phosphorylation of human p53 protein determined by mass spectrometry." in: Biochemistry, Vol. 40, Issue 13, pp. 4053-66, (2001) (PubMed).

    Lu, Fisher, Bailey, Levine: "The CDK7-cycH-p36 complex of transcription factor IIH phosphorylates p53, enhancing its sequence-specific DNA binding activity in vitro." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5923-34, (1997) (PubMed).

    Lohrum, Scheidtmann: "Differential effects of phosphorylation of rat p53 on transactivation of promoters derived from different p53 responsive genes." in: Oncogene, Vol. 13, Issue 12, pp. 2527-39, (1997) (PubMed).

  • Target

    p53 (TP53) (Tumor Protein P53 (TP53))

    Alternative Name

    P53

    Pathways

    p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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