FLT4 antibody (Extracellular Domain)

Catalog No. ABIN967368

Product Details anti-FLT4 Antibody

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Binding Specificity: Extracellular Domain

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FLT4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: VEGFR-3 extracellular domain

Clone: AFL4

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistochemistry:
The AFL4 antibody, specific for mouse VEGFR-3, is tested for immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed and formalin-fixed, paraffin-embedded sections. Antigen retrieval with Retrievagen A is recommended for formalin-fixed, paraffin sections. Tissues tested were mouse spleen, liver, kidney, lung and small intestine. The antibody stains endothelial cells of lymphatic vessels. The isotype control recommended for use with this antibody is purified rat IgG2akappa. For optimal indirect immunohistochemical staining, the AFL4 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-rat Igs (multiple adsorbed) as the secondary antibody and Streptavidin-HRP together with the DAB Detection System. More conveniently, the Anti-Rat Ig HRP Detection Kit, which contains the biotinylated secondary antibody, antibody diluent, streptavidin-HRP and DAB substrate, can be used for staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-FLT4 antibody (ABIN967368)

Stacker, Caesar, Baldwin, Thornton, Williams, Prevo, Jackson, Nishikawa, Kubo, Achen: "VEGF-D promotes the metastatic spread of tumor cells via the lymphatics." in: Nature medicine, Vol. 7, Issue 2, pp. 186-91, (2001) (PubMed).

Kubo, Fujiwara, Jussila, Hashi, Ogawa, Shimizu, Awane, Sakai, Takabayashi, Alitalo, Yamaoka, Nishikawa: "Involvement of vascular endothelial growth factor receptor-3 in maintenance of integrity of endothelial cell lining during tumor angiogenesis." in: Blood, Vol. 96, Issue 2, pp. 546-53, (2000) (PubMed).

Target Details for FLT4

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Alternative Name: VEGFR-3 (FLT4 Products)

Synonyms: FLT41 antibody, LMPH1A antibody, PCL antibody, VEGFR3 antibody, AI323512 antibody, Chy antibody, Flt-4 antibody, VEGFR-3 antibody, Vegfr3 antibody, cb1059 antibody, fms related tyrosine kinase 4 antibody, FMS-like tyrosine kinase 4 antibody, fms-related tyrosine kinase 4 antibody, FLT4 antibody, Flt4 antibody, flt4 antibody

Background: The AFL4 antibody reacts with mouse VEGFR-3, an endothelial-specific receptor tyrosine kinase (RTK) related to VEGF receptors. The immunogen used was the extracellular domain of VEGFR-3, corresponding to nucleotides 45-2354 in the GeneBank L07296. VEGFR-3 is induced in all endothelial cells (EC’s) during early embryogenesis, and its expression eventually disappears from the vascular endothelial cells of adult tissues. VEGFR-3 is constitutively expressed in the adult lymphatic endothelium. Although VEGFR-3 is not expressed in adult blood vessels, it is induced in vascular endothelial cells of tumor-bearing tissues. Studies have shown that both VEGF-C and VEGF-D, ligands for VEGFR-3, promote lymphatic vessel proliferation throughout central tumor areas and at their peripheries, demonstrating that such molecules aid in the spread of tumor cells from the primary tumor to local lymph nodes.

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling